# Publications We are committed to sharing data in a timely manner with the community. Below we list selected relevant non-peer-reviewed preprints that provide immediate access during the review process, as well as peer-reviewed publications. Download 21 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=50&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=ris) ### 2026 Matthew Butnaru, William McKenna, Srishti Goswami, Alejandra Wu-Chuang, Enzo Mameli, Abigail Wilcox, Leopoldine Quennesson, Ah-Ram Kim, Austin Veal, Weihang Chen, Hugo Verzone, Elizabeth A Lane, Hanna J Laukaitis-Yousey, Chad Araneo, Nisha Singh, Joao Pedra, Yanhui Hu, Raghuvir Viswanatha, Norbert Perrimon, and Stephanie E Mohr. 2026. “[Genome-Wide CRISPR Knockout Cell Screening Platform for the Disease Vector Tick Species Ixodes Scapularis](/publication/genome-wide-crispr-knockout-cell-screening-platform-disease-vector-tick-species-ixodes)”. BioRxiv. doi:10.64898/2026.05.05.721418 Matthew Butnaru, William McKenna, Srishti Goswami, Alejandra Wu-Chuang, Enzo Mameli, Abigail Wilcox, Leopoldine Quennesson, Ah-Ram Kim, Austin Veal, Weihang Chen, Hugo Verzone, Elizabeth A Lane, Hanna J Laukaitis-Yousey, Chad Araneo, Nisha Singh, Joao Pedra, Yanhui Hu, Raghuvir Viswanatha, Norbert Perrimon, and Stephanie E Mohr. 2026. “[Genome-Wide CRISPR Knockout Cell Screening Platform for the Disease Vector Tick Species Ixodes Scapularis](/publication/genome-wide-crispr-knockout-cell-screening-platform-disease-vector-tick-species-ixodes)”. BioRxiv. doi:10.64898/2026.05.05.721418 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2026/05/07/2026.05.05.721418) The black legged tick, Ixodes scapularis, is a vector of the bacterium that causes Lyme disease and several other illnesses, including anaplasmosis, babesiosis, and tick-borne encephalitis. Although high-quality genome annotations are available for I... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2026/05/07/2026.05.05.721418) ### 2025 Raghuvir Viswanatha, Donghoon Lee, William R. Robins, Enzo Mameli, Yanhui Hu, Ah-Ram Kim, Yousuf Hashmi, Hiroshi Nishida, Gyan Prakash, Matthew Butnaru, Sterling Churchman, Stephanie E. Mohr, John J. Mekalanos, and Norbert Perrimon. 2025. “[A Family of Lethal Exotoxins Defined by Cell Entry via the Attractin Receptor](/publication/family-lethal-exotoxins-defined-cell-entry-attractin-receptor)”. BioRxiv. doi:10.1101/2025.10.08.681221 Raghuvir Viswanatha, Donghoon Lee, William R. Robins, Enzo Mameli, Yanhui Hu, Ah-Ram Kim, Yousuf Hashmi, Hiroshi Nishida, Gyan Prakash, Matthew Butnaru, Sterling Churchman, Stephanie E. Mohr, John J. Mekalanos, and Norbert Perrimon. 2025. “[A Family of Lethal Exotoxins Defined by Cell Entry via the Attractin Receptor](/publication/family-lethal-exotoxins-defined-cell-entry-attractin-receptor)”. BioRxiv. doi:10.1101/2025.10.08.681221 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/10/11/2025.10.08.681221.full.pdf) Although bacterial genomes encode numerous potential toxins, it is unclear how evolution drives the specificity of these important virulence factors. Using an insect CRISPR screen, we identified the transmembrane protein Attractin (ATRN) as the receptor... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/10/11/2025.10.08.681221.full.pdf) Muhammad Ahmad, Raghuvir Viswanatha, Ah-Ram Kim, and Norbert Perrimon. 2025. “[A Genome-Wide CRISPR Screen Reveals ZDHHC8-Dependent Gαq Palmitoylation As a Key Regulator of GPCR Signaling](/publication/genome-wide-crispr-screen-reveals-zdhhc8-dependent-gaq-palmitoylation-key-regulator)”. BioRxiv. doi:10.1101/2025.08.06.668953 Muhammad Ahmad, Raghuvir Viswanatha, Ah-Ram Kim, and Norbert Perrimon. 2025. “[A Genome-Wide CRISPR Screen Reveals ZDHHC8-Dependent Gαq Palmitoylation As a Key Regulator of GPCR Signaling](/publication/genome-wide-crispr-screen-reveals-zdhhc8-dependent-gaq-palmitoylation-key-regulator)”. BioRxiv. doi:10.1101/2025.08.06.668953 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/08/06/2025.08.06.668953.full.pdf) G protein-coupled receptors (GPCRs) that couple to the Gαq signaling pathway control diverse physiological processes, yet the full complement of cellular regulators for this pathway remains unknown. Here, we report the first genome-wide CRISPR knockout... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/08/06/2025.08.06.668953.full.pdf) Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3 Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3 - add\_circle do\_not\_disturb\_on Abstract CRISPR screens enable systematic, scalable genotype-to-phenotype mapping. We previously developed a CRISPR screening method for Drosophila melanogaster and mosquito cell lines using plasmid transfection and site-specific integration to introduce single... Michele Marconcini, Steeve Cruchet, Srishti Goswami, Raghuvir Viswanatha, Matthew Butnaru, Joydeep De, Camilla Roselli, Dafni Hadjieconomou, Norbert Perrimon, Stephanie E. Mohr, and Richard Benton. 2025. “[Intersecting Experimental Evolution and CRISPR Screens to Identify Novel Toxin Resistance Loci](/publication/intersecting-experimental-evolution-and-crispr-screens-identify-novel-toxin-resistance)”. BioRxiv. doi:10.1101/2025.07.23.666417 Michele Marconcini, Steeve Cruchet, Srishti Goswami, Raghuvir Viswanatha, Matthew Butnaru, Joydeep De, Camilla Roselli, Dafni Hadjieconomou, Norbert Perrimon, Stephanie E. Mohr, and Richard Benton. 2025. “[Intersecting Experimental Evolution and CRISPR Screens to Identify Novel Toxin Resistance Loci](/publication/intersecting-experimental-evolution-and-crispr-screens-identify-novel-toxin-resistance)”. BioRxiv. doi:10.1101/2025.07.23.666417 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/12/02/2025.07.23.666417.full.pdf) Understanding toxin resistance in insects is key to appreciate niche adaptations, but remains challenging due to its often complex genetic basis. A well-known example is the specialized association of Drosophila sechellia with noni fruit (Morinda... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2025/12/02/2025.07.23.666417.full.pdf) Yanhui Hu, Aram Comjean, Jonathan Rodiger, Weihang Chen, Ah-Ram Kim, Mujeeb Qadiri, Chenxi Gao, Jonathan Zirin, Stephanie E Mohr, and Norbert Perrimon. 2025. “[FlyRNAi.Org 2025 Update-Expanded Resources for New Technologies and Species.](/publication/flyrnaiorg-2025-update-expanded-resources-new-technologies-and-species)”. Nucleic Acids Research, 53, D1, Pp. D958-D965. doi:10.1093/nar/gkae917 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Weihang Chen, Ah-Ram Kim, Mujeeb Qadiri, Chenxi Gao, Jonathan Zirin, Stephanie E Mohr, and Norbert Perrimon. 2025. “[FlyRNAi.Org 2025 Update-Expanded Resources for New Technologies and Species.](/publication/flyrnaiorg-2025-update-expanded-resources-new-technologies-and-species)”. Nucleic Acids Research, 53, D1, Pp. D958-D965. doi:10.1093/nar/gkae917 - add\_circle do\_not\_disturb\_on Abstract The design, analysis and mining of large-scale 'omics studies with the goal of advancing biological and biomedical understanding require use of a range of bioinformatics tools, including approaches tailored to needs specific to a given species and/or... ### 2024 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 - add\_circle do\_not\_disturb\_on Abstract mosquitoes are the sole vector of human malaria, the most burdensome vector-borne disease worldwide. Strategies aimed at reducing mosquito populations and limiting their ability to transmit disease show the most promise for disease control. Therefore... ### 2023 Yanhui Hu, Aram Comjean, Helen Attrill, Giulia Antonazzo, Jim Thurmond, Weihang Chen, Fangge Li, Tiffany Chao, Stephanie E Mohr, Nicholas H Brown, and Norbert Perrimon. 2023. “[PANGEA: A New Gene Set Enrichment Tool for Drosophila and Common Research Organisms.](/publication/pangea-new-gene-set-enrichment-tool-drosophila-and-common-research-organisms)”. Nucleic Acids Research, 51, W1, Pp. W419-W426. doi:10.1093/nar/gkad331 Yanhui Hu, Aram Comjean, Helen Attrill, Giulia Antonazzo, Jim Thurmond, Weihang Chen, Fangge Li, Tiffany Chao, Stephanie E Mohr, Nicholas H Brown, and Norbert Perrimon. 2023. “[PANGEA: A New Gene Set Enrichment Tool for Drosophila and Common Research Organisms.](/publication/pangea-new-gene-set-enrichment-tool-drosophila-and-common-research-organisms)”. Nucleic Acids Research, 51, W1, Pp. W419-W426. doi:10.1093/nar/gkad331 - add\_circle do\_not\_disturb\_on Abstract Gene set enrichment analysis (GSEA) plays an important role in large-scale data analysis, helping scientists discover the underlying biological patterns over-represented in a gene list resulting from, for example, an 'omics' study. Gene Ontology (GO)... Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie E Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “[Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research.](/publication/continuous-muscle-glial-epithelial-neuronal-and-hemocyte-cell-lines-research)”. ELife, 12. doi:10.7554/eLife.85814 Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie E Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “[Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research.](/publication/continuous-muscle-glial-epithelial-neuronal-and-hemocyte-cell-lines-research)”. ELife, 12. doi:10.7554/eLife.85814 - add\_circle do\_not\_disturb\_on Abstract Expression of activated Ras, Ras, provides cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted Ras expression to generate continuous cell lines of muscle... Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie E Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells.](/publication/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. ELife, 12. doi:10.7554/eLife.85542 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie E Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells.](/publication/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. ELife, 12. doi:10.7554/eLife.85542 - add\_circle do\_not\_disturb\_on Abstract Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes... ### 2022 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor.](/publication/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor.](/publication/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 - add\_circle do\_not\_disturb\_on Abstract Entomopathogenic nematodes are widely used as biopesticides. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors. No protein receptors are known... Hans M Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys R Berman, Rebekah Rushforth, Stephanie E Mohr, Norbert Perrimon, and Clement Y Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress.](/publication/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genetics, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 Hans M Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys R Berman, Rebekah Rushforth, Stephanie E Mohr, Norbert Perrimon, and Clement Y Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress.](/publication/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genetics, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 - add\_circle do\_not\_disturb\_on Abstract Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1-CDG is caused by mutations in the gene encoding the first step in N-glycosylation... Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie E Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila.](/publication/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends in Genetics : TIG, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie E Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila.](/publication/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends in Genetics : TIG, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 - add\_circle do\_not\_disturb\_on Abstract For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many genetic and physiological similarities to humans and the availability of sophisticated... ### 2021 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M Colpitts, Stephanie E Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos.](/publication/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. Nature Communications, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M Colpitts, Stephanie E Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos.](/publication/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. Nature Communications, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 - add\_circle do\_not\_disturb\_on Abstract Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured mosquito cells. Moreover, application in mammalian cells of forward genetic approaches such as... ### 2020 Baolong Xia, Gabriel Amador, Raghuvir Viswanatha, Jonathan Zirin, Stephanie E Mohr, and Norbert Perrimon. 2020. “[CRISPR-Based Engineering of Gene Knockout Cells by Homology-Directed Insertion in Polyploid Drosophila S2R+ Cells.](/publication/crispr-based-engineering-gene-knockout-cells-homology-directed-insertion-polyploid)”. Nature Protocols, 15, 10, Pp. 3478-98. doi:10.1038/s41596-020-0383-8 Baolong Xia, Gabriel Amador, Raghuvir Viswanatha, Jonathan Zirin, Stephanie E Mohr, and Norbert Perrimon. 2020. “[CRISPR-Based Engineering of Gene Knockout Cells by Homology-Directed Insertion in Polyploid Drosophila S2R+ Cells.](/publication/crispr-based-engineering-gene-knockout-cells-homology-directed-insertion-polyploid)”. Nature Protocols, 15, 10, Pp. 3478-98. doi:10.1038/s41596-020-0383-8 - add\_circle do\_not\_disturb\_on Abstract Precise and efficient genome modifications provide powerful tools for biological studies. Previous CRISPR gene knockout methods in cell lines have relied on frameshifts caused by stochastic insertion/deletion in all alleles. However, this method is... ### 2019 Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie E Mohr, and Norbert Perrimon. 2019. “[Pooled CRISPR Screens in Drosophila Cells.](/publication/pooled-crispr-screens-drosophila-cells)”. Current Protocols in Molecular Biology, 129, 1, Pp. e111. doi:10.1002/cpmb.111 Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie E Mohr, and Norbert Perrimon. 2019. “[Pooled CRISPR Screens in Drosophila Cells.](/publication/pooled-crispr-screens-drosophila-cells)”. Current Protocols in Molecular Biology, 129, 1, Pp. e111. doi:10.1002/cpmb.111 - add\_circle do\_not\_disturb\_on Abstract High-throughput screens in Drosophila melanogaster cell lines have led to discovery of conserved gene functions related to signal transduction, host-pathogen interactions, ion transport, and more. CRISPR/Cas9 technology has opened the door to new types of... ### 2018 Naoki Okamoto, Raghuvir Viswanatha, Riyan Bittar, Zhongchi Li, Sachiko Haga-Yamanaka, Norbert Perrimon, and Naoki Yamanaka. 2018. “[A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila.](/publication/membrane-transporter-required-steroid-hormone-uptake-drosophila)”. Developmental Cell, 47, 3, Pp. 294-305.e7. doi:10.1016/j.devcel.2018.09.012 Naoki Okamoto, Raghuvir Viswanatha, Riyan Bittar, Zhongchi Li, Sachiko Haga-Yamanaka, Norbert Perrimon, and Naoki Yamanaka. 2018. “[A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila.](/publication/membrane-transporter-required-steroid-hormone-uptake-drosophila)”. Developmental Cell, 47, 3, Pp. 294-305.e7. doi:10.1016/j.devcel.2018.09.012 - add\_circle do\_not\_disturb\_on Abstract Steroid hormones are a group of lipophilic hormones that are believed to enter cells by simple diffusion to regulate diverse physiological processes through intracellular nuclear receptors. Here, we challenge this model in Drosophila by demonstrating that... Raghuvir Viswanatha, Zhongchi Li, Yanhui Hu, and Norbert Perrimon. 2018. “[Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Cells.](/publication/pooled-genome-wide-crispr-screening-basal-and-context-specific-fitness-gene)”. ELife, 7. doi:10.7554/eLife.36333 Raghuvir Viswanatha, Zhongchi Li, Yanhui Hu, and Norbert Perrimon. 2018. “[Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Cells.](/publication/pooled-genome-wide-crispr-screening-basal-and-context-specific-fitness-gene)”. ELife, 7. doi:10.7554/eLife.36333 - add\_circle do\_not\_disturb\_on Abstract Genome-wide screens in cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site... Raghuvir Viswanatha, Zhongchi Li, Yanhui Hu, and Norbert Perrimon. 2018. “[Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Cells.](/publication/pooled-genome-wide-crispr-screening-basal-and-context-specific-fitness-gene-0)”. ELife, 7. doi:10.7554/eLife.36333 Raghuvir Viswanatha, Zhongchi Li, Yanhui Hu, and Norbert Perrimon. 2018. “[Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Cells.](/publication/pooled-genome-wide-crispr-screening-basal-and-context-specific-fitness-gene-0)”. ELife, 7. doi:10.7554/eLife.36333 - add\_circle do\_not\_disturb\_on Abstract Genome-wide screens in cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site... ### 2016 Stephanie E Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin E Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “[CRISPR Guide RNA Design for Research Applications.](/publication/crispr-guide-rna-design-research-applications)”. The FEBS Journal, 283, 17, Pp. 3232-8. doi:10.1111/febs.13777 Stephanie E Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin E Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “[CRISPR Guide RNA Design for Research Applications.](/publication/crispr-guide-rna-design-research-applications)”. The FEBS Journal, 283, 17, Pp. 3232-8. doi:10.1111/febs.13777 - add\_circle do\_not\_disturb\_on Abstract The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on-target and effective guide RNAs (gRNAs). Here, we review the state... Stephanie E Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin E Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “[CRISPR Guide RNA Design for Research Applications.](/publication/crispr-guide-rna-design-research-applications-0)”. The FEBS Journal, 283, 17, Pp. 3232-8. doi:10.1111/febs.13777 Stephanie E Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin E Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “[CRISPR Guide RNA Design for Research Applications.](/publication/crispr-guide-rna-design-research-applications-0)”. The FEBS Journal, 283, 17, Pp. 3232-8. doi:10.1111/febs.13777 - add\_circle do\_not\_disturb\_on Abstract The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on-target and effective guide RNAs (gRNAs). Here, we review the state... [ View All Citations arrow\_circle\_right ](/publications)